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MONTANA
VETERINARY
DIAGNOSTIC
LABORATORY
PHONE: (406) 994-4885
FAX: (406) 994-6344
WEBSITE: www.liv.mt.gov/lab
WEB RESULTS: https://mvdl.mt.gov
E-MAIL: mvdl@mt.gov
1911 WEST LINCOLN STREET
BOZEMAN, MT 59718
NECROPSY KIT
PURPOSE
The necropsy kit is designed for routine postmortem collection of tissues for diseases other than abortions and neonatal diarrheas (specific kits are available for these circumstances), as improved collection and preservation of samples in the field will greatly facilitate laboratory analysis. The information and supplies provided can be useful for sample collection and submission in all species.
COLLECTION OVERVIEW
The most common error is to fail to collect a complete tissue set because of a preconceived diagnosis.
Another common error is to collect only tissues that appear grossly abnormal. Many disease processes can only be identified by microscopic examination. Therefore, collect both normal and abnormal tissues from all recommended sites.
TEST ORDER & INTERPRETATION OVERVIEW
A necropsy workup begins with histologic examination, which has the potential to guide further testing. If desired, additional testing can be specifically requested either at initial submission or following histologic evaluation and pathologist consultation.
SPECIMEN COLLECTION (LISTED IN ORDER OF IMPORTANCE), KIT CONTENTS & USE
Samples to collect:
Primary: heart, lung, liver, kidney spleen, brain, stomach, small intestine and colon
As indicated: lymph nodes, adrenal gland, thyroid gland, pancreas, gastrointestinal contents, other
Single animal submission form and large Whirl-Pak® bag
Bottle of 10% neutral buffered formalin (NBF) and large Biohazard bag Fixed Tissues:
Two small and one large Whirl-Pak\circledR bags
Complete the submission form – include history
Place completed form back into the Whirl-Pak® bag and close bag to prevent contamination during transport
Tissues should not be greater than 1/2 inch thick
Immediately place tissue in 10% NBF (the volume ratio of NBF to tissue should be at least 10 to 1)
Cap tightly (no tape please) and place bottle in Biohazard bag with absorbency labeled with animal ID
Fresh Tissues:
Tissues should be in approximately 2 to 3-inch cubes
Label each bag with animal ID, tissues contained, and Owner's name
Place labeled, closed small bags into a large Whirl-Pak\circledR bag and close large bag
The sealed Whirl-Pak® bags should be packed with frozen ice packs inside an absorbent filled plastic bag
LIVE ANIMAL SUBMISSION
Submission of live animals for necropsy is not typically encouraged and should always be prearranged with the laboratory.
SHIPPING (ALL CARRIERS)
Montana Veterinary Diagnostic Lab
1911 West Lincoln Street
Marsh Lab, Room 76
Bozeman, MT 59718-4132
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