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Montana Veterinary Diagnostic Laboratory Salmonella Enteritidis (SE) Sample Submission 1911 West Lincoln Street Bozeman, MT 59718-4132

Web Portal: https://mvdl.mt.gov Email: mvdl@mt.gov Website: http://liv.mt.gov/Diagnostic-Lab Phone 406-994-4885 Fax 406-994-6344

Lab Use Only: Clear Form

Owner/Submitter: ___ Billing Address: ___ Address: ___

<table><tr><td>Copy of Report To:</td><td>Colony</td><td>Wilcox</td><td>Other:</td><td></td></tr><tr><td></td><td>Fax</td><td>Email</td><td>Other:</td><td></td></tr><tr><td></td><td>Fax/Email/#:</td><td></td><td></td><td></td></tr></table>

Phone: ___ Email: ___

Fax: ___ Other: ___

Ranch/Collection Site: ___

Sample Profile: Chick Papers Pullets Weeks: ___ Pre-Production Weeks: ___ Mid-Production (Layers) Weeks: ___ Push Outs Weeks: ___ Other: ___

Collection Date/Time: ___

Sampled By (please print/type): ___

Sampled By (signature affixed): ___

Number/Type Specimens: ___

Flock ID: _____ House/Barn ID: _____ Copy of Report To: Colony Wilcox Other: _____ Fax Email Other: _____ Fax/Email/#: _____

Test Procedure Requested (choose one): PCR Culture

Sample ID Number: Lab Use Only:

F:\User\QMS Form\System\Submission Form\5.7.2.3 MVDL Salmonella Enteritidis Submission Form.docx\ Revision Date 3-22-21

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COLLECTION

1. Disinfect the top of a can of evaporated milk and open with a disinfected can opener
2. Moisten the drag swab with evaporated milk and collect the sample
3. Place each drag swab in a separate 18 ounce Whirl-Pak™ bag containing sufficient evaporated milk (about 1 tablespoon) to keep the swab moist in transit An 18 ounce bag enables us to add test materials to your sample
4. PLEASE DO NOT use small sandwich bags, snack bags, or an excess of evaporated milk
4. Press the air out of the Whirl-Pak™ bag and seal by twirling the bag three times while holding the wire end tabs, or folding the end over tightly three times
5. Bend the wire tabs over the bag edges to hold the seal
6. Make sure the outside of the Whirl-Pak™ bag is clean, dry and clearly labeled to correspond to the SE Submission Form
7. Please use a separate SE Submission Form for each barn sampled
7. Place all sealed Whirl-Pak™ bags from a barn in a gallon Ziploc® bag
8. Specify PCR or Culture test on SE Submission Form
9. Complete the SE Submission Form and place it in a separate, clean Ziplock® bag to prevent contamination of the paperwork if a leak should occur
10. Keep the samples cool (40°F – 45°F) during collection if sample collection requires more than 6 hours

Label Whirl-Pak™ bag

- Remove Perforation/ Add milk/ Add swab - Twirl to close - Press tabs to seal

SHIPPING

1. Place samples in a shipping container with frozen gel packs to keep samples cool (40°F – 45°F)
2. Ideally, samples should be shipped the same day as collected and received by MVDL within 48 hours
3. Ship to: MVDL, 1911 West Lincoln, Bozeman, MT 59718 via priority overnight delivery (FedEx or UPS)
4. Note: discounted flat rate shipping is available through our web portal; please log in at https://mvdl.mt.gov or contact us for more information
4. Notify MVDL by calling 406-994-4885 with shipment date and number of samples in shipment
5. For best turn-around-time, ship on Monday and Tuesday
6. If chick paper collection occurs on a Friday, refrigerate samples and ship on Monday

Do not ship samples on Friday or Saturday

RECEIPT AND TESTING OF SAMPLES

1. Upon arrival, MVDL will assess and record transit time and temperature to verify that samples are acceptable for testing
2. MVDL will notify the submitter prior to testing if sample quality or sample identification is questionable

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