Hobart mixer; extruded into spaghetti-like strands; air-dried using drying racks for 24 hours; grinded up into small shrimp pellets and sieved; oils were then added and then stored in the freezer until fed. Juvenile shrimp were fed four (4) feedings per day (8:00am, 11:15am, 3:15pm, 6pm) at 15 percent of total shrimp body weight and adjusted higher or lower based upon feeding activity.

Dissolved oxygen, salinity, pH, and water temperature were measured daily using a Hydrolab Quanta Water Quality Handheld Monitoring System: Model QD 02152, while total ammonia nitrogen and nitrite levels were recorded twice weekly. To account for water-loss from evaporation and routine maintenance (siphoning), approximately 5 percent of the total water volume was replaced daily. Tanks were siphoned daily to remove leftover feed and feces.

Growth and feed utilization parameters were calculated as follows:

  • ✓ Body mass gain (\%) = [(Final body mass - initial body mass)/Initial body mass] x 100
  • ✓ Specific growth rate (%/day) = [((ln final body mass in g) - (ln initial body mass in g))/number of trial days] x 100
  • ✓ Feed conversion ratio (FCR) = dry feed fed (g)/body mass gain (g)
  • ✓ Protein efficiency ratio = body mass gain (g)/crude protein fed (g)
  • ✓ Lipid efficiency ratio = body mass gain (g)/crude lipid fed (g)

Results

Table 2: Data from table below demonstrates that water quality parameters were within optimal range for Pacific white shrimp.

15-Jun-1516-Jun-1517-Jun-1518-Jun-1519-Jun-1520-Jun-15
Temp (°C)29.2629.2128.928.8428.8228.79
pH6.047.467.57.857.82
DO (ppm)4.154.284.14.13.55
Salinity (ppt)25.826.327.5128.0828.1628.23
Nitrite (ppm)0.675
TAN (ppm)0.27

Initial weight, final weight, and percent survival parameters are presented in Table 3 showing positive results for those shrimp fed algal oil in combination with other