Hobart mixer; extruded into spaghetti-like strands; air-dried using drying racks for 24 hours; grinded up into small shrimp pellets and sieved; oils were then added and then stored in the freezer until fed. Juvenile shrimp were fed four (4) feedings per day (8:00am, 11:15am, 3:15pm, 6pm) at 15 percent of total shrimp body weight and adjusted higher or lower based upon feeding activity.
Dissolved oxygen, salinity, pH, and water temperature were measured daily using a Hydrolab Quanta Water Quality Handheld Monitoring System: Model QD 02152, while total ammonia nitrogen and nitrite levels were recorded twice weekly. To account for water-loss from evaporation and routine maintenance (siphoning), approximately 5 percent of the total water volume was replaced daily. Tanks were siphoned daily to remove leftover feed and feces.
Growth and feed utilization parameters were calculated as follows:
✓ Body mass gain (\%) = [(Final body mass - initial body mass)/Initial body mass] x 100
✓ Specific growth rate (%/day) = [((ln final body mass in g) - (ln initial body mass in g))/number of trial days] x 100
✓ Feed conversion ratio (FCR) = dry feed fed (g)/body mass gain (g)
✓ Protein efficiency ratio = body mass gain (g)/crude protein fed (g)
✓ Lipid efficiency ratio = body mass gain (g)/crude lipid fed (g)
Table 2: Data from table below demonstrates that water quality parameters were within optimal range for Pacific white shrimp.
| 15-Jun-15 | 16-Jun-15 | 17-Jun-15 | 18-Jun-15 | 19-Jun-15 | 20-Jun-15 |
|---|
| Temp (°C) | 29.26 | 29.21 | 28.9 | 28.84 | 28.82 | 28.79 |
|---|
| pH | 6.04 | 7.46 | 7.5 | 7.85 | 7.82 | |
|---|
| DO (ppm) | 4.15 | 4.28 | 4.1 | 4.1 | 3.55 | |
|---|
| Salinity (ppt) | 25.8 | 26.3 | 27.51 | 28.08 | 28.16 | 28.23 |
|---|
| Nitrite (ppm) | | | 0.675 | | | |
|---|
| TAN (ppm) | | | 0.27 | | | |
|---|
Initial weight, final weight, and percent survival parameters are presented in Table 3 showing positive results for those shrimp fed algal oil in combination with other