Fig. 1. ISSR based UPGMA dendrogram of Nei's (1978) genetic distance showing genetic similarity among 19 pawpaw (Asimina triloba) cultivars.
plan to identify additional and more reproducible ISSR markers to accurately fingerprint all pawpaw cultivars and separate remaining cultivar genetic identities.
In conclusion, seven primers yielded 11 Mendelian markers with either a 3:1 or 1:1 ratio that was confirmed by chi-square analysis. Using 10 ISSR Mendelian markers, an analysis of genetic diversity with leaf DNA samples collected from 19 pawpaw cultivars revealed a moderate to high level of genetic diversity in the selections. These data suggest that ISSR marker methodology has a higher level of discrimination in evaluating genetic diversity in pawpaw than do isozyme or RAPD marker systems.
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