Example of Array Tomography with a mouse brain tissue sample. Large overview images were recorded at a resolution sufficient for detection of synapses in multiple sections; synapses were mapped with pan & zoom in the Maps Software, and high-resolution imaging was performed in sub-areas of multiple sections.
Examples of volume renderings of a zebrafish embryo head (left) and of zebrafish embryo eye tissue (right). Both samples were imaged in low-vacuum mode using the DBS detector. The zebrafish embryo head was imaged at 2 kV (100 pA) with 3 μs dwelltime. The total volume is 350 μm × 350 μm × 83 μm using a total of 829 images (with a slice thickness of 100 nm and voxel size of 42 nm × 42 nm × 100 nm). The images required 7 days of continuous imaging. Scale bar: 100 μm. The zebrafish embryo eye tissue was imaged at 3 kV (100 pA) with 3 μs dwelltime. The total volume is 136 μm × 94 μm × 31 μm using a total of 772 images (with a slice thickness of 40 nm and voxel size of 48 nm × 48 nm × 40 nm). Courtesy for both samples: Robert Creton, Brown University. Scale bar: 20 μm
3D imaging applications Volume rendering using the Volumescope System