A bout nine grams of paw paw pulp was extracted with ethanol and concentrated through a series of methanol and chloroform extractions as described by Ratnayake et al., (1993).
Twenty brine shrimp larvae, taken 48 h after initiation of hatching in artificial sea water were added to each vial (5mL) containing 0, 10, 100 and 1000 ppm concentrations of extract.
A after 24 h, survivors were counted. SD from three extracts.