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COURSE OBJECTIVES:

This course is to provide in-depth knowledge about Biotechnology, plus a complementary laboratory sessions to gain hands-on skills. The theoretical aspects and basic principles are consolidated by conducting hands-on laboratory experiments commonly used in Biotechnology. The economic, business, and aspects on environmental impact will also be discussed.

STUDENT LEARNING OUTCOMES:

1. Understand the basic principles in Biotechnology
2. Have a broad perspective on Biotechnology: What is it, how does it work, how it affects society and economic impact
3. Have acquired hands-on skills in several basic techniques involved in Biotechnology:
4. a.DNA manipulations: Restriction endonuclease digestion and ligation of DNA in the construction of expression vectors, transformation, purification, and electrophoresis
5. b.Protein manipulations: SDS-PAGE, western blot hybridization, ELISA, fluorescence protein analysis, etc.
6. c.Prokaryotic and eukaryotic cell culture and manipulations: bacterial subculture, animal cell culture and passage, microscopy including florescence microscopy
7. d.Chromatography and other purification technologies
4. Understand the principles of scaling up/industrial manufacturing/distribution process
5. Financial aspects/structures, intellectual protection, scalability of Biotechnology, and career potential/path for graduates
6. Tour of regional biotech companies [when and if available]

REQUIRED TEXTS:

1. Biotechnology: Science for the New Millennium Revised Edition
2. Laboratory Manual for Biotechnology and Laboratory Science: The Basics

BLACKBOARD:

Blackboard will be used to provide announcements, lecture materials (notes and presentations), assignments, and miscellaneous information for the course. Students should make sure that the email address listed for them in Blackboard is up-to-date since the instructor may use Blackboard-based email to provide additional information or announcements to students. Also, Smart Thinking provides 24/7 tutorial assistance.

COURSE SPECIFIC REQUIREMENTS, EXPECTATIONS, POLICIES:

Attendance: Attendance is a major predictor for classroom success! Attendance will be taken by a sign-in sheet or roll call. Be sure your attendance has been noted before you leave class. One hundred (100) points are allocated for perfect attendance: If you have 100% attendance (excluding documented excuses), you earn the 100 points, 90% and 90 points, and so on.

Excessive absences will result in a grade penalty (see KSU Bulletin). Students will not be allowed to make up work missed due to non-excused absence. Students more than 15 minutes late to lab or class without a valid reason will be marked absent. In the event of an unexpected absence, make every effort to contact the instructor before, or as soon after the absence as is possible. Also see, "Student Handbook." http://kysu.edu/administration-governance/student-affairs/student-handbook/

Laboratory Periods: Laboratory periods will be spent using a variety of resources – A-V presentations, computer simulations, hands-on experimentation, etc. – to complement the topics discussed in lectures. Each laboratory period may begin with a short 5 to 10-minute Quiz. Students late to the lab will either miss the quiz entirely or will have a reduced time to complete the quiz. Special Requirement: each person in the laboratory is required to wear a lab coat.