Agilent Technologies

Agilent Technologies, Inc. 5301 Stevens Creek Blvd Santa Clara, CA 95051 United States

The Agilent Fragment Analyzer Systems - Unique Specifications and Facts

In the market, only the Agilent Fragment Analyzer systems provide:

1. Large sample handling capability

  • Able to load and analyze at least three 96-well sample plates (288 samples) unattended without any user intervention or additional robotics/liquid handling requirement

2. Continuous illumination and detection of capillaries

  • Has continuous LED illumination of the capillaries AND continuous uninterrupted detection of fluorescent signal through a CCD Detector
  • Uses LED based fluorescence with excitation at 470 nm and detection at 520-580 nm

3. Uninterrupted operations

  • Able to change 96-well sample plates without the need for stopping in-progress instrument operation

4. Ability to use different capillary arrays

  • Utilizes a capillary array format with the ability to use capillary arrays of different lengths (22cm, 33cm and 55cm)

5. Ability to switch between gel types

  • Able to switch between 2 different gel/application types without stopping in-progress instrument operation

6. High sensitivity

  • Has demonstrated sensitivity for fragments of at least 5 pg/μL and demonstrated resolution to 3bp for fragments under 300 bp

7. High resolution

  • Has a sizing accuracy of ≤ 5% and sizing precision of ≤ 2%

8. A wide sample application range

  • Able to use qualitative kit and quantitative kits
  • Able to analyze dsDNA fragments from 35 bp to 50,000 bp, total RNA, messenger RNA, small RNAs, genomic DNA and DNA smears

9. Integrated data analysis package

  • Has a data analysis software package capable of sizing, quantification, smear analysis, integrity determination and report generation

10. Automated operations

  • Has automated flushing of the capillaries between every injection cycle
  • Able to perform simultaneous, parallel injection and separation of at least 12 samples at once from a 96-well sample plate, and sample from all the wells of the plate