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"text": "however, their isozyme study included 15 advanced selections from the PawPaw Foundation breeding effort and 2 cultivars that were not included in this ISSR study. Huang et al. (2000) used 21 RAPD markers that segregated in a simple Mendelain fashion and used 6 populations (10 to 18 trees each) from 6 states in the KSU repository collection to evaluate genetic diversity. Direct comparisons of the effectiveness of all three marker systems would require the use of these marker systems with the same genotypes. However, it would appear that the 10 ISSR loci had a higher level of polymorphism than the nine isozyme markers of Huang et al. (1997). Previous studies did not support the idea that high levels of genetic variation could be maintained in A. triloba, due to its clonal reproductive habit and low seed set in nature (Rogstad et al., 1991). This study provides additional evidence that moderate to high levels of genetic diversity exist in A. triloba (Huang et al. 1997, 1998, 2000)."
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"text": "were uniquely identified (Fig. 1). The ISSR based UPGMA den-\nrogram showing genetic similarity among 19 pawpaw cultivars\nusing separation by Dice (1945) was very similar to the separation\nusing Nei’s (1978) genetic distance (data not shown). The ISSR\nseparation confirms the findings of Huang et al. (1997, 2003), in\nthat the cultivar NC-1, which has been thought to be a seedling of\na ‘Davis’ x ‘Oversleese’ cross, does not share many markers with\nthese cultivars. A mix up of pollen or seed must have occurred\nand NC-1 may have a different parentage than that reported. The\ncultivar IXL, which is thought to be a seedling of an ‘Overleese’ x\n‘Davis’ cross, did show some genetic similarity with these parents.\nGenetic relationships for the remaining cultivars in this study were\nsimilar to those found by Huang et al. with isozymes (1997) and\nRAPD markers (2003). The pawpaw cultivars SAA-Zimmerman\nand Mitchell, Prolific and Taytwo, and Sweet Alice and SAA-Zim-\nmerman, shared similar grouping in both the dendrograms of Huang\net al. (2003) (Fig. 1). Additional ISSR Mendelian markers could\nfurther improve genetic diversity estimates in pawpaw cultivars. We"
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"text": "The ISSR markers could be useful tools in cultivar identification. Using Nei’s (1978) genetic distance, 11 of the 19 cultivars were uniquely identified (Fig. 1). The ISSR based UPGMA den-\nprogram showing genetic similarity among 19 pawpaw cultivars\nusing separation by Dice (1945) was very similar to the separation\nusing Nei’s (1978) genetic distance (data not shown). The ISSR\nseparation confirms the findings of Huang et al. (1997, 2003), in\nthat the cultivar NC-1, which has been thought to be a seedling of\na ‘Davis’ x ‘Oversleese’ cross, does not share many markers with\nthese cultivars. A mix up of pollen or seed must have occurred\nand NC-1 may have a different parentage than that reported. The\ncultivar IXL, which is thought to be a seedling of an ‘Overleese’ x\n‘Davis’ cross, did show some genetic similarity with these parents.\nGenetic relationships for the remaining cultivars in this study were\nsimilar to those found by Huang et al. with isozymes (1997) and\nRAPD markers (2003). The pawpaw cultivars SAA-Zimmerman\nand Mitchell, Prolific and Taytwo, and Sweet Alice and SAA-Zim-\nmerman, shared similar grouping in both the dendrograms of Huang\net al. (2003) (Fig. 1). Additional ISSR Mendelian markers could\nfurther improve genetic diversity estimates in pawpaw cultivars. We"
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"text": "J. AMER. SOC. HORT. SCI. 128(4):521–525. 2003.",
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